Label
Recombinant DNA principles and methodologies, edited by James J. Greene, Venigalla B. Rao
Bibliography note
Incluye referencias bibliográficas e índices
Illustrations
illustrations
Main title
Recombinant DNA principles and methodologies
Nature of contents
bibliography
Responsibility statement
edited by James J. Greene, Venigalla B. Rao
Table of contents
Why cloning? / James J. Greene -- Biochemistry of nucleic acids / Paul S. Miller -- Enzymology of recombinant DNA / Frank J. Castora -- Basic Escherichia coli genetics useful for recombinant DNA techniques / Darren D. Sledjeski and Susan Garges -- Formulation of cloning strategies / John B. Dame -- Isolation and purification of nucleic acids / James J. Greene -- Gel electrophoresis, southern and northern analysis / James J. Greene and Frank J. Castora -- Hybridization probes / Frank J. Castora and James J. Greene -- cDNA cloning and the construction of recombinant DNA / Dante S. Zarlenga -- Molecular cloning by plasmid vectors / Paulina Balbás and Francisco Bolívar -- Phage lambda: basic biology and use as a vector / Susan Frackman and Michael Feiss -- Cosmid cloning / John Collins and Werner Lindenmaier -- Multipurpose cloning vectors / August J. Sick, Joseph M. Fernandez, and Jay M. Short -- Mammalian expression vectors / Ricky R. Hirschhorn and Nava Sarver -- Approaches to the expression of foreign genes / Allan R. Shatzman -- Screening for specific recombinant clones / Lucía Irene González-Villaseñor and Mark M. Manak -- Principles and applications of the polymerase chain reaction / Gabriela S. Dveksler and Carl W. Dieffenbach -- DNA sequencing / Maurice A. Kashdan ... [et al.] -- Site-directed mutagenesis / Alfred C. Johnson and Marvin Reitz